A11_Determination of alkylpyrrolidone (NMP, NEP) metabolites in urine

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Last Updated: 13-11-2018 14:46


In this theoretical training course, the determination of urinary N-methyl-2-pyrrolidone (NMP) and N-ethyl-2-pyrrolidone (NEP) metabolites (2-hydroxy-N-methylsuccinimide (2-HMSI), and 5-Hydroxy-N-methyl-2-pyrrolidone (5-HNMP) in case of NMP and 2-hydroxy-N-ethylsuccinimide (2-HESI) and 5-Hydroxy-N-ethyl-2-pyrrolidone (5-HNEP) in case of NEP) via GC-EI-MS/MS after solid phase extraction (SPE) and derivatization (silylation) is presented. The analysis procedure is explained and critical steps and pitfalls are pointed out. Exemplary chromatograms are shown and the quantitative analysis is explained. Finally, exemplary studies are presented.